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4x laemli loading buffer  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 4x laemli loading buffer
    4x Laemli Loading Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemli+loading+buffer/4x+laemli+loading+buffer/pmc08890653-92-5-29
    Average 90 stars, based on 1 article reviews
    4x laemli loading buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Cell-penetrating peptides containing the progesterone receptor polyproline domain inhibits EGF signaling and cell proliferation in lung cancer cells
    Article Snippet: .. Cell lysates were mixed with 4x Laemli loading buffer, loaded on 8% SDS-PAGE gels and transferred onto PVDF membranes.Membranes were probed with primary antibodies recognizing phospho-p44/42 MAPK (1:1000 v/v, Cell Signaling Technology, USA) and total MAPK antibody (1:1000 v/v, Cell Signaling Technology, USA) overnight at 4°C. .. Next, blots were incubated with an anti-rabbit secondary antibody (1:5000 v/v in 5%BSA-TBST) for 1 h at room temperature and visualized by chemiluminescence using Pierce® ECL Immunoblotting Substrate (Thermo Scientific, USA).



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